By Dongyou Liu
As extra unique molecular protocols and next variations are defined within the literature, it has turn into tough for these ultimately serious about the improvement of those protocols to grasp that are correct to undertake for actual id of bacterial pathogens. Molecular Detection of Human Bacterial Pathogens addresses this factor, with foreign scientists in respective bacterial pathogen examine and analysis offering specialist summaries on present diagnostic techniques for significant human bacterial pathogens.
Each bankruptcy contains a quick assessment at the class, epidemiology, scientific gains, and analysis of an immense pathogenic bacterial genus, an summary of scientific pattern assortment and practise strategies, a variety of consultant stepwise molecular protocols, and a dialogue on additional study necessities on the subject of greater analysis.
This publication represents a competent and handy reference on molecular detection and id of significant human bacterial pathogens; an critical instrument for upcoming and skilled clinical, veterinary, and commercial laboratory scientists engaged in bacterial characterization; and a necessary textbook for undergraduate and graduate scholars in microbiology.
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Additional resources for Molecular Detection of Human Bacterial Pathogens
Yersinia pestis was responsible for the most fatal and devastating bacterial disease in history—the bubonic plague—which killed an estimated 200 million people prior to the advent of antibiotics. Currently, the most common fatal bacterial diseases are respiratory infections, with tuberculosis (caused by Mycobacterium tuberculosis) alone killing about 2 million people a year. One of the world’s deadliest bacterial diseases today is cholera, which is caused by foodborne Vibrio cholerae. Other globally important bacterial diseases include pneumonia caused by Streptococcus and Pseudomonas, tetanus, typhoid fever, diphtheria, syphilis, and leprosy.
Similarly, a negative result by a molecular assay for a given pathogen normally indicates the absence of the pathogen. 8 However, it is equally important to rule out the possibility of false negative results. One possible cause is due to the low sensitivity of the assay employed. Alternatively, an insufficient amount of bacteria may be present in the sample (due to sample degradation or prior antibiotic treatment). Another cause may be the impurity of the processed sample. 20–24 Any impurities and contaminations present in the samples after nucleic acid isolation may contribute to false negative results.
Liu, D. Microbiol. Methods, 71, 133, 2007. 11. G. , Biotechnical use of polymerase chain reaction for microbiological analysis of biological samples, Biotechnol. Annu. , 5, 87, 2000. 12. , Molecular methods for the assessment of bacterial viability, J. Microbiol. Methods, 53, 175, 2003. 13. , Identification, subtyping and virulence determination of Listeria monocytogenes, in important foodborne pathogen, J. Med. , 55, 645, 2006. 14. , Nucleic acid-based methods for the detection of bacterial pathogens: Present and future considerations for the clinical laboratory, Clin.
Molecular Detection of Human Bacterial Pathogens by Dongyou Liu