By John Swansbury
A set of key cytogenetic and FISH concepts utilized by glossy scientific laboratories within the genetic research of human malignancies. The book's useful recommendation and techniques are appropriate to be used at each point of workmanship, together with absolutely verified laboratories, yet with a sympathetic bias in the direction of an individual contemplating constructing a brand new cytogenetics provider. right here the reader will locate not just easy tutorials at the basics of human karyotypes and chromosome research, but in addition precise discussions on how laboratories might optimally improve their repertoire of features to incorporate such more moderen complementary suggestions as CGH, FISH, and M-FISH.
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Additional resources for Cancer Cytogenetics: Methods and Protocols (Methods in Molecular Biology)
Webber, L. M. and Garson, O. M. (1983) Fluorodeoxyuridine synchronisation of bone marrow cultures. Cancer Genet. Cytogenet. 8, 123–132. The Myeloid Disorders 23 3 The Myeloid Disorders Background John Swansbury 1. Introduction Malignant myeloid disorders have broadly similar responses to cytogenetic techniques and many have similar chromosome abnormalities. Included are diseases that are frankly malignant, such as acute myeloid leukemia (AML), and some that may be regarded as premalignant, such as the myeloproliferative disorders (MPD).
All the chromosome abnormalities that occur in MDS and MPD also occur in AML, although in different proportions. However, there are some abnormalities that occur in AML that are extremely rare in other disorders, including t(8;21)(q22;q22), t(15;17)(q24;q21), and 32 Swansbury inv(16)(p13q22). It may be no coincidence that these abnormalities are generally confined to granulocytic cells and are associated with a good prognosis, while most other abnormalities tend to occur in all kinds of myeloid cells and are broadly associated with a poorer prognosis.
However, if secondary abnormalities are detected during the course of the disease, then this is a stronger indication that acceleration of the disease is imminent. Cytogenetic studies of large numbers of divisions have shown that in some cases these late-appearing abnormalities were present at diagnosis, but at a very low incidence (B. Reeves, unpublished observations). The introduction of FISH The Myeloid Disorders 27 Fig. 1. Examples of recurrent abnormalities in myeloid disorders, particularly illustrating some that can be subtle.
Cancer Cytogenetics: Methods and Protocols (Methods in Molecular Biology) by John Swansbury